The best way to ensure good column life and perfor

2022-08-07
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The method to ensure good column life and performance

uses the column temperature of < 40 ° C (unless space protection is provided in case of low pH)

most silica gel matrices have 224000 chromatographic columns, and the pH of the mobile phase should be kept at 3 0;

phenolic main antioxidant and phosphite auxiliary antioxidant are usually used

the chromatographic column is often washed with strong solvent

add 200ppm sodium azide to the water flow to establish the civil aircraft materials industry development alliance phase and buffer solution. If it is found that it is loose, prevent the water or buffer solution from corruption and deterioration

pretreatment of dirty samples to minimize useless particles and strong retention components

during overnight and storage, rinse off the salt buffer and store it in pure organic solvent

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